π-HuB will dissect the human body into a hierarchy of digital reference space of the proteome. That is, by harnessing the rapid-evolving techniques like single-cell and spatial proteomics, to digitalize the complete quantitative composition of a human body, including the cell composition of all major tissues/organs, the protein composition of individual cell types and single cells, and the proteome-centric molecular networks within cells (e.g., proteoform crosstalks, protein complexes, protein-protein interactions, DNA/RNA-protein interactions, protein-metabolite interactions).